Peptide synthesis and purification

The core facility offers peptide synthesis as a service employing the so-called "solid-phase peptide synthesis (SPPS)¹". Standard Fmoc chemistry is primarily used for the preparation of peptides and mini-proteins containing proteinogenic, modified-proteinogenic and non-proteinogenic amino acids.

The synthesis services cover both manual and automated techniques. For the automated synthesis, the following devices are employed:

Core Facility homepage ²

Protein synthesis and purification ³

Bioanalytical methods for the characterization of peptides and proteins ⁴

Economy Peptide Synthesizer EPS 221 (Intavis) & ResPep SL (Intavis)

Purification of the raw peptides is achieved by preparative HPLC (example):

verschiedene Säulen (C4, C8, C18, 250 x 30 mm, 250 x 22 mm, 250 x 4,6 mm) SL (Intavis)

Different amounts ranging from 1-200 mg can be applied.

Requests involving complex synthesis procedures such as controlled closure of disulfide connectivities, postsynthetic modifications of amino acids/peptides, native chemical ligation and others can be performed after individual consultation².

Peptide synthesis and purification

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